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CD104

Order Information
Catalog# Size/Concentration Price
31347 1.0mg/ml $341.00
Specification
Entrez GeneID 3691
Aliases ITGB4; GP150
Clone# 5G3G5
MW 202.2kDa
Host/Isotype Mouse IgG2a
Storage 4℃; -20℃ for long term storage
Species Reactivity Human;
Immunogen Purified recombinant fragment of human CD104 (AA: extra 29-206) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
Description
Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
References
1.Sci Rep. 2015 Nov 17;5:16529.
2.Acta Derm Venereol. 2015 Jan;95(1):112-3.
Sequence
extra(29-206)
Related Products

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD104 mAb against human CD104 (AA: extra 29-206) recombinant protein. (Expected MW is 46.5 kDa)

Western Blot

Figure 3:Western blot analysis using CD104 mAb against HEK293 (1) and CD104 (AA: extra 29-206)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using CD104 mouse mAb against A549 (1), A431 (2), and SW620 (3) cell lysate.

Flow cytometric

Figure 5:Flow cytometric analysis of HL-60 cells using CD104 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CD104 mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer -
B1/2 Esophageal Cancer +
C1/2 Esophagus -
D1/2 Esophagus -
E1/2 Stomach Cancer -
F1/2 Stomach Cancer -
G1/2 Stomach -
H1/2 Stomach -
A3/4 Colon Cancer -
B3/4 Colon Cancer -
C3/4 Colon -
D3/4 Duodenum -
E3/4 Rectal Cancer +
F3/4 Rectal Cancer +
G3/4 Rectum -
H3/4 Testis -
A5/6 Hepatic Carcinoma -
B5/6 Hepatic Carcinoma -
C5/6 Liver -
D5/6 Renal cell Carcinoma -
E5/6 Kidney -
F5/6 Pancreas -
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue -
A7/8 Lung Cancer -
B7/8 Lung Cancer -
C7/8 Lung -
D7/8 Brain -
E7/8 Brain -
F7/8 Medulla oblongata -
G7/8 Cerebellar -
H7/8 Skeletal muscle -
A9/10 Breast Cancer -
B9/10 Breast Cancer -
C9/10 Ovarian Cancer -
D9/10 Ovarian Cancer -
E9/10 Endometrial Cancer -
F9/10 Cervical Cancer -
G9/10 Bladder Cancer -
H9/10 Myocardial -
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