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CD166

Order Information
Catalog# Size/Concentration Price
31343 1.0mg/ml $341.00
Specification
Entrez GeneID 214
Aliases MEMD; ALCAM
Clone# 2F1B12
MW 65kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human;
Immunogen Purified recombinant fragment of human CD166 (AA: extra 227-381) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
Description
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
References
1.Diagn Pathol. 2015 Jul 2;10:86.
2.Asian Pac J Cancer Prev. 2015;16(9):3849-56.
Sequence
extra(227-381)
Related Products

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD166 mAb against human CD166 (AA: extra 227-381) recombinant protein. (Expected MW is 47 kDa)

Western Blot

Figure 3:Western blot analysis using CD166 mAb against HEK293 (1) and CD166 (AA: extra 227-381)-hIgGFc transfected HEK293 (2) cell lysate.

Flow cytometric

Figure 4:Flow cytometric analysis of HL-60 cells using CD166 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CD166 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD166 mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer +
B1/2 Esophageal Cancer +
C1/2 Esophagus +
D1/2 Esophagus -
E1/2 Stomach Cancer +
F1/2 Stomach Cancer +
G1/2 Stomach -
H1/2 Stomach -
A3/4 Colon Cancer +
B3/4 Colon Cancer -
C3/4 Colon -
D3/4 Duodenum -
E3/4 Rectal Cancer -
F3/4 Rectal Cancer +
G3/4 Rectum -
H3/4 Testis -
A5/6 Hepatic Carcinoma +
B5/6 Hepatic Carcinoma +
C5/6 Liver +
D5/6 Renal cell Carcinoma -
E5/6 Kidney +
F5/6 Pancreas +
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue -
A7/8 Lung Cancer +
B7/8 Lung Cancer -
C7/8 Lung -
D7/8 Brain -
E7/8 Brain -
F7/8 Medulla oblongata -
G7/8 Cerebellar -
H7/8 Skeletal muscle -
A9/10 Breast Cancer -
B9/10 Breast Cancer +
C9/10 Ovarian Cancer +
D9/10 Ovarian Cancer +
E9/10 Endometrial Cancer +
F9/10 Cervical Cancer +
G9/10 Bladder Cancer +
H9/10 Myocardial -
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