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Order Information
Catalog# Size/Concentration Price
31343 1.0mg/ml $341.00
Entrez GeneID 214
Clone# 2F1B12
MW 65kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human;
Immunogen Purified recombinant fragment of human CD166 (AA: extra 227-381) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
1.Diagn Pathol. 2015 Jul 2;10:86.
2.Asian Pac J Cancer Prev. 2015;16(9):3849-56.
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Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD166 mAb against human CD166 (AA: extra 227-381) recombinant protein. (Expected MW is 47 kDa)

Western Blot

Figure 3:Western blot analysis using CD166 mAb against HEK293 (1) and CD166 (AA: extra 227-381)-hIgGFc transfected HEK293 (2) cell lysate.

Flow cytometric

Figure 4:Flow cytometric analysis of HL-60 cells using CD166 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CD166 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD166 mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer +
B1/2 Esophageal Cancer +
C1/2 Esophagus +
D1/2 Esophagus -
E1/2 Stomach Cancer +
F1/2 Stomach Cancer +
G1/2 Stomach -
H1/2 Stomach -
A3/4 Colon Cancer +
B3/4 Colon Cancer -
C3/4 Colon -
D3/4 Duodenum -
E3/4 Rectal Cancer -
F3/4 Rectal Cancer +
G3/4 Rectum -
H3/4 Testis -
A5/6 Hepatic Carcinoma +
B5/6 Hepatic Carcinoma +
C5/6 Liver +
D5/6 Renal cell Carcinoma -
E5/6 Kidney +
F5/6 Pancreas +
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue -
A7/8 Lung Cancer +
B7/8 Lung Cancer -
C7/8 Lung -
D7/8 Brain -
E7/8 Brain -
F7/8 Medulla oblongata -
G7/8 Cerebellar -
H7/8 Skeletal muscle -
A9/10 Breast Cancer -
B9/10 Breast Cancer +
C9/10 Ovarian Cancer +
D9/10 Ovarian Cancer +
E9/10 Endometrial Cancer +
F9/10 Cervical Cancer +
G9/10 Bladder Cancer +
H9/10 Myocardial -
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