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BTRC

Order Information
Catalog# Size/Concentration Price
31282 1.0mg/ml $341.00
Specification
Entrez GeneID 8945
Aliases FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP
Clone# 3D5E6
MW 68.9kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human;
Immunogen Purified recombinant fragment of human BTRC (AA: 24-151) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
ELISA 1/10000
Description
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6.
References
1.Biochem Biophys Res Commun. 2013 Nov 29;441(4):831-7.
2.PLoS One. 2011;6(11):e27464.
Sequence
24-151
Related Products

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using BTRC mAb against human BTRC (AA: 24-151) recombinant protein. (Expected MW is 40.2 kDa)

Western Blot

Figure 3:Western blot analysis using BTRC mAb against HEK293 (1) and BTRC (AA: 24-151)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using BTRC mouse mAb against Ramos (1), MCF-7 (2), and K562 (3) cell lysate.

Immunohistochemical analysis

Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using BTRC mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer -
B1/2 Esophageal Cancer -
C1/2 Esophagus -
D1/2 Esophagus -
E1/2 Stomach Cancer -
F1/2 Stomach Cancer -
G1/2 Stomach -
H1/2 Stomach -
A3/4 Colon Cancer -
B3/4 Colon Cancer +
C3/4 Colon -
D3/4 Duodenum -
E3/4 Rectal Cancer +
F3/4 Rectal Cancer +
G3/4 Rectum -
H3/4 Testis -
A5/6 Hepatic Carcinoma -
B5/6 Hepatic Carcinoma -
C5/6 Liver -
D5/6 Renal cell Carcinoma -
E5/6 Kidney -
F5/6 Pancreas -
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue -
A7/8 Lung Cancer -
B7/8 Lung Cancer -
C7/8 Lung -
D7/8 Brain -
E7/8 Brain -
F7/8 Medulla oblongata -
G7/8 Cerebellar -
H7/8 Skeletal muscle -
A9/10 Breast Cancer -
B9/10 Breast Cancer -
C9/10 Ovarian Cancer -
D9/10 Ovarian Cancer -
E9/10 Endometrial Cancer -
F9/10 Cervical Cancer -
G9/10 Bladder Cancer -
H9/10 Myocardial -
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