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CTNNB1

Order Information
Catalog# Size/Concentration Price
31277 1.0mg/ml $341.00
Specification
Entrez GeneID 1499
Aliases CTNNB; MRD19; armadillo
Clone# 7C5A2
MW 85.5kDa
Host/Isotype Mouse IgG2b
Storage 4℃; -20℃ for long term storage
Species Reactivity Human; Mouse;
Immunogen Purified recombinant fragment of human CTNNB1 (AA: 1-100) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
Description
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
References
1.Anticancer Res. 2016 Apr;36(4):1599-604.
2.Int J Clin Exp Pathol. 2015 Nov 1;8(11):14989-94.
Sequence
1-100
Related Products

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CTNNB1 mAb against human CTNNB1 (AA: 1-100) recombinant protein. (Expected MW is 37.1 kDa)

Western Blot

Figure 3:Western blot analysis using CTNNB1 mAb against HEK293 (1) and CTNNB1 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using CTNNB1 mouse mAb against Hela (1), SH-SY5Y (2), NIH/3T3 (3), and HEK293(4) cell lysate.

Flow cytometric

Figure 5:Flow cytometric analysis of Hela cells using CTNNB1 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CTNNB1 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CTNNB1 mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer +
B1/2 Esophageal Cancer +
C1/2 Esophagus +
D1/2 Esophagus -
E1/2 Stomach Cancer +
F1/2 Stomach Cancer +
G1/2 Stomach +
H1/2 Stomach -
A3/4 Colon Cancer +
B3/4 Colon Cancer +
C3/4 Colon +
D3/4 Duodenum +
E3/4 Rectal Cancer +
F3/4 Rectal Cancer +
G3/4 Rectum +
H3/4 Testis +
A5/6 Hepatic Carcinoma -
B5/6 Hepatic Carcinoma -
C5/6 Liver -
D5/6 Renal cell Carcinoma -
E5/6 Kidney -
F5/6 Pancreas -
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue -
A7/8 Lung Cancer -
B7/8 Lung Cancer +
C7/8 Lung -
D7/8 Brain -
E7/8 Brain -
F7/8 Medulla oblongata -
G7/8 Cerebellar -
H7/8 Skeletal muscle -
A9/10 Breast Cancer +
B9/10 Breast Cancer -
C9/10 Ovarian Cancer +
D9/10 Ovarian Cancer -
E9/10 Endometrial Cancer +
F9/10 Cervical Cancer +
G9/10 Bladder Cancer +
H9/10 Myocardial -
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