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ATP2A1

Order Information
Catelog# Size/Concentration Price
31211 1.0mg/ml $341.00
Specification
Entrez GeneID 487
Aliases ATP2A; SERCA1
Clone# 3D1H6
MW 110kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human; Mouse; Monkey;
Immunogen Purified recombinant fragment of human ATP2A1 (AA: 487-631) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
FCM 1/200 - 1/400
ELISA 1/10000
Description
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms.
References
1.Biochem Biophys Res Commun. 2012 Jun 29;423(2):212-7.
2.Mol Genet Metab. 2013 Sep-Oct;110(1-2):162-9.
Sequence
487-631
Related Products

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using ATP2A1 mAb against human ATP2A1 (AA: 487-631) recombinant protein. (Expected MW is 42 kDa)

Western Blot

Figure 3:Western blot analysis using ATP2A1 mAb against HEK293 (1) and ATP2A1 (AA: 487-631)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using ATP2A1 mouse mAb against C2C12 (1), COS7 (2), Hela (3), K562 (4), and Jurkat (5) cell lysate.

Flow cytometric

Figure 5:Flow cytometric analysis of HeLa cells using ATP2A1 mouse mAb (green) and negative control (red).