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SMARCA1

Order Information
Catelog# Size/Concentration Price
31205 1.0mg/ml $341.00
Specification
Entrez GeneID 6594
Aliases SWI; ISWI; SWI2; SNF2L; SNF2L1; SNF2LB; SNF2LT; hSNF2L; NURF140
Clone# 2H7B9
MW 122.6kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human; Mouse;
Immunogen Purified recombinant fragment of human SMARCA1 (AA: 933-1070) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
Description
This gene encodes a member of the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription. The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells. Alternative splicing results in multiple transcript variants.
References
1.Yonsei Med J. 2013 May 1;54(3):772-7.
2.BMC Med Genet. 2008 Feb 26;9:11.
Sequence
933-1070
Related Products

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using SMARCA1 mAb against human SMARCA1 (AA: 933-1070) recombinant protein. (Expected MW is 42.4 kDa)

Western Blot

Figure 3:Western blot analysis using SMARCA1 mAb against HEK293 (1) and SMARCA1 (AA: 933-1070)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using SMARCA1 mouse mAb against PANC-1 (1), HEK293 (2), SW620 (3), HT-29 (4), SH-SY5Y (5), and SK-OV-3 (6) cell lysate.

Flow cytometric

Figure 5:Flow cytometric analysis of NIH/3T3 cells using SMARCA1 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using SMARCA1 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using SMARCA1 mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer +
B1/2 Esophageal Cancer +
C1/2 Esophagus -
D1/2 Esophagus -
E1/2 Stomach Cancer +
F1/2 Stomach Cancer -
G1/2 Stomach +
H1/2 Stomach -
A3/4 Colon Cancer +
B3/4 Colon Cancer -
C3/4 Colon -
D3/4 Duodenum -
E3/4 Rectal Cancer -
F3/4 Rectal Cancer +
G3/4 Rectum -
H3/4 Testis -
A5/6 Hepatic Carcinoma +
B5/6 Hepatic Carcinoma +
C5/6 Liver -
D5/6 Renal cell Carcinoma -
E5/6 Kidney -
F5/6 Pancreas -
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue -
A7/8 Lung Cancer -
B7/8 Lung Cancer +
C7/8 Lung -
D7/8 Brain -
E7/8 Brain -
F7/8 Medulla oblongata -
G7/8 Cerebellar -
H7/8 Skeletal muscle -
A9/10 Breast Cancer -
B9/10 Breast Cancer -
C9/10 Ovarian Cancer -
D9/10 Ovarian Cancer -
E9/10 Endometrial Cancer -
F9/10 Cervical Cancer -
G9/10 Bladder Cancer -
H9/10 Myocardial -
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