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APEX1

Order Information
Catelog# Size/Concentration Price
31203 1.0mg/ml $341.00
Specification
Entrez GeneID 328
Aliases APE; APX; APE1; APEN; APEX; HAP1; REF1
Clone# 7A2G7
MW 35.6kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human; Monkey; Rat;
Immunogen Purified recombinant fragment of human APEX1 (AA: 219-318) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
Description
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.
References
1.Mutat Res Genet Toxicol Environ Mutagen. 2015 Nov;793:19-29.
2.PLoS One. 2015 Dec 1;10(12):e0143289.
Sequence
219-318
Related Products

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using APEX1 mAb against human APEX1 (AA: 219-318) recombinant protein. (Expected MW is 37.4 kDa)

Western Blot

Figure 3:Western blot analysis using APEX1 mAb against HEK293 (1) and APEX1 (AA: 219-318)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using APEX1 mouse mAb against Hela (1), Jurkat (2), SW480 (3), A431 (4), HepG2 (5), NIH/3T3 (6), and PC-12 (7) cell lysate.

Flow cytometric

Figure 5:Flow cytometric analysis of HeLa cells using APEX1 mouse mAb (green) and negative control (red).

Flow cytometric

Figure 6:Flow cytometric analysis of SK-N-SH cells using APEX1 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using APEX1 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 8:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using APEX1 mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer +
B1/2 Esophageal Cancer +
C1/2 Esophagus +
D1/2 Esophagus +
E1/2 Stomach Cancer +
F1/2 Stomach Cancer -
G1/2 Stomach +
H1/2 Stomach +
A3/4 Colon Cancer +
B3/4 Colon Cancer +
C3/4 Colon +
D3/4 Duodenum +
E3/4 Rectal Cancer +
F3/4 Rectal Cancer +
G3/4 Rectum -
H3/4 Testis -
A5/6 Hepatic Carcinoma +
B5/6 Hepatic Carcinoma +
C5/6 Liver +
D5/6 Renal cell Carcinoma -
E5/6 Kidney +
F5/6 Pancreas +
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue +
A7/8 Lung Cancer +
B7/8 Lung Cancer +
C7/8 Lung -
D7/8 Brain -
E7/8 Brain +
F7/8 Medulla oblongata -
G7/8 Cerebellar +
H7/8 Skeletal muscle -
A9/10 Breast Cancer +
B9/10 Breast Cancer +
C9/10 Ovarian Cancer +
D9/10 Ovarian Cancer +
E9/10 Endometrial Cancer +
F9/10 Cervical Cancer +
G9/10 Bladder Cancer +
H9/10 Myocardial -
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