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ESR1

Order Information
Catalog# Size/Concentration Price
31155 1.0mg/ml $341.00
Specification
Entrez GeneID 2099
Aliases ER; ESR; Era; ESRA; ESTRR; NR3A1
Clone# 6A6F3
MW 66.2kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human;
Immunogen Purified recombinant fragment of human ESR1 (AA: 2-185) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
ICC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
Description
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined.
References
1.Tumour Biol. 2015 Aug;36(8):6349-59.
2.Breast Cancer Res. 2014 Dec 12;16(6):494.
Sequence
2-185
Related Products

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using ESR1 mAb against human ESR1 (AA: 2-185) recombinant protein. (Expected MW is 45.8 kDa)

Western Blot

Figure 3:Western blot analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), and Hela (6) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using ESR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunofluorescence analysis

Figure 7:Immunofluorescence analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Figure 7:Flow cytometric analysis of Hela cells using ESR1 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 8:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using ESR1 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 9:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ESR1 mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer +
B1/2 Esophageal Cancer +
C1/2 Esophagus +
D1/2 Esophagus +
E1/2 Stomach Cancer +
F1/2 Stomach Cancer +
G1/2 Stomach +
H1/2 Stomach -
A3/4 Colon Cancer +
B3/4 Colon Cancer +
C3/4 Colon +
D3/4 Duodenum +
E3/4 Rectal Cancer +
F3/4 Rectal Cancer +
G3/4 Rectum +
H3/4 Testis +
A5/6 Hepatic Carcinoma +
B5/6 Hepatic Carcinoma +
C5/6 Liver +
D5/6 Renal cell Carcinoma +
E5/6 Kidney +
F5/6 Pancreas +
G5/6 Prostate Cancer +
H5/6 Lymphoid tissue +
A7/8 Lung Cancer +
B7/8 Lung Cancer +
C7/8 Lung +
D7/8 Brain +
E7/8 Brain +
F7/8 Medulla oblongata +
G7/8 Cerebellar +
H7/8 Skeletal muscle -
A9/10 Breast Cancer +
B9/10 Breast Cancer +
C9/10 Ovarian Cancer +
D9/10 Ovarian Cancer +
E9/10 Endometrial Cancer +
F9/10 Cervical Cancer +
G9/10 Bladder Cancer +
H9/10 Myocardial -
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