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Order Information
Catalog# Size/Concentration Price
31133 1.0mg/ml $341.00
Entrez GeneID 3858
Aliases BIE; EHK; K10; KPP; BCIE; CK10
Clone# 3C2F5
MW 58.8kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human; Mouse; Monkey; Rat;
Immunogen Purified recombinant fragment of human KRT10 (AA: 345-454 ) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.
1.JAMA Dermatol. 2015 Jan;151(1):64-9.
2.Histopathology. 2012 Nov;61(5):910-20.
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Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using KRT10 mAb against human KRT10 (AA: 345-454) recombinant protein. (Expected MW is 38.7 kDa)

Western Blot

Figure 3:Western blot analysis using KRT10 mAb against HEK293 (1) and KRT10 (AA: 345-454)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using KRT10 mouse mAb against A431 (1), C6 (2), COS7 (3), Jurkat (4), NIH/3T3 (5), and HEK293 (6) cell lysate.

Flow cytometric

Figure 5:Flow cytometric analysis of Hela cells using KRT10 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using KRT10 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using KRT10 mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer +
B1/2 Esophageal Cancer +
C1/2 Esophagus -
D1/2 Esophagus -
E1/2 Stomach Cancer -
F1/2 Stomach Cancer -
G1/2 Stomach -
H1/2 Stomach -
A3/4 Colon Cancer -
B3/4 Colon Cancer -
C3/4 Colon -
D3/4 Duodenum -
E3/4 Rectal Cancer -
F3/4 Rectal Cancer +
G3/4 Rectum -
H3/4 Testis -
A5/6 Hepatic Carcinoma +
B5/6 Hepatic Carcinoma +
C5/6 Liver -
D5/6 Renal cell Carcinoma -
E5/6 Kidney -
F5/6 Pancreas -
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue -
A7/8 Lung Cancer -
B7/8 Lung Cancer -
C7/8 Lung -
D7/8 Brain -
E7/8 Brain +
F7/8 Medulla oblongata -
G7/8 Cerebellar -
H7/8 Skeletal muscle -
A9/10 Breast Cancer +
B9/10 Breast Cancer +
C9/10 Ovarian Cancer +
D9/10 Ovarian Cancer -
E9/10 Endometrial Cancer -
F9/10 Cervical Cancer -
G9/10 Bladder Cancer -
H9/10 Myocardial -
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