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KRT19

Order Information
Catelog# Size/Concentration Price
31131 1.0mg/ml $341.00
Specification
Entrez GeneID 3880
Aliases K19; CK19; K1CS
Clone# 8H1A8
MW 44.1kDa
Host/Isotype Mouse IgG2a
Storage 4℃; -20℃ for long term storage
Species Reactivity Human;
Immunogen Purified recombinant fragment of human KRT19 (AA: 274-400) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
ELISA 1/10000
Description
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.
References
1.Clin Cancer Res. 2015 Jul 1;21(13):3081-91.
2.Gut. 2014 Apr;63(4):674-85.
Sequence
274-400
Related Products

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using KRT19 mAb against human KRT19 (AA: 274-400) recombinant protein. (Expected MW is 40 kDa)

Western Blot

Figure 3:Western blot analysis using KRT19 mAb against HEK293 (1) and KRT19 (AA: 274-400)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using KRT19 mouse mAb against MCF-7 (1), SW480 (2), SW620 (3), and HCT116 (4) cell lysate.

Immunohistochemical analysis

Figure 5:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using KRT19 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using KRT19 mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer +
B1/2 Esophageal Cancer +
C1/2 Esophagus +
D1/2 Esophagus +
E1/2 Stomach Cancer +
F1/2 Stomach Cancer +
G1/2 Stomach +
H1/2 Stomach -
A3/4 Colon Cancer +
B3/4 Colon Cancer +
C3/4 Colon +
D3/4 Duodenum +
E3/4 Rectal Cancer +
F3/4 Rectal Cancer +
G3/4 Rectum +
H3/4 Testis -
A5/6 Hepatic Carcinoma +
B5/6 Hepatic Carcinoma +
C5/6 Liver +
D5/6 Renal cell Carcinoma -
E5/6 Kidney +
F5/6 Pancreas +
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue -
A7/8 Lung Cancer +
B7/8 Lung Cancer +
C7/8 Lung -
D7/8 Brain -
E7/8 Brain +
F7/8 Medulla oblongata -
G7/8 Cerebellar -
H7/8 Skeletal muscle -
A9/10 Breast Cancer +
B9/10 Breast Cancer +
C9/10 Ovarian Cancer +
D9/10 Ovarian Cancer +
E9/10 Endometrial Cancer +
F9/10 Cervical Cancer +
G9/10 Bladder Cancer +
H9/10 Myocardial -
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