1.866.339.0871         info@promab.com  info@promab.com

MMP2

Order Information
Catelog# Size/Concentration Price
31126 1.0mg/ml $341.00
Specification
Entrez GeneID 4313
Aliases CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
Clone# 2B11E6
MW 73.8kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human; Mouse;
Immunogen Purified recombinant fragment of human MMP2 (AA: 9-140) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
FCM 1/200 - 1/400
ELISA 1/10000
Description
This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
References
1.Reprod Biol Endocrinol. 2015 Sep 4;13:102.
2.PLoS One. 2015 Mar 27;10(3):e0121404.
Sequence
9-140
Related Products

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using MMP2 mAb against human MMP2 (AA: 9-140) recombinant protein. (Expected MW is 42.2 kDa)

Western Blot

Figure 3:Western blot analysis using MMP2 mAb against HEK293 (1) and MMP2 (AA: 9-140)-hIgGFc transfected HEK293 (2) cell lysate.

Flow cytometric

Figure 4:Flow cytometric analysis of Hela cells using MMP2 mouse mAb (green) and negative control (red).

Western Blot

Figure 5:Western blot analysis using MMP2 mouse mAb against MCF-7 (1), Raw264.7 (2), HUVEC (3), and T47D (4) cell lysate.