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UHRF1

Order Information
Catalog# Size/Concentration Price
31125 1.0mg/ml $341.00
Specification
Entrez GeneID 29128
Aliases Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95
Clone# 1A1B1
MW 89.8kDa
Host/Isotype Mouse IgG2b
Storage 4℃; -20℃ for long term storage
Species Reactivity Human;
Immunogen Purified recombinant fragment of human UHRF1 (AA: 616-755) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
ICC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
Description
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.
References
1.Biomarkers. 2015;20(3):183-8.
2.Med Oncol. 2013 Dec;30(4):613.
Sequence
616-755
Related Products

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using UHRF1 mAb against human UHRF1 (AA: 616-755) recombinant protein. (Expected MW is 41.8 kDa)

Western Blot

Figure 3:Western blot analysis using UHRF1 mAb against HEK293 (1) and UHRF1 (AA: 616-755)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using UHRF1 mouse mAb against MCF-7 (1) and Hela (2) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using UHRF1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunofluorescence analysis

Figure 6:Immunofluorescence analysis of Hela cells using UHRF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Figure 7:Flow cytometric analysis of MCF-7 cells using UHRF1 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using UHRF1 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 9:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using UHRF1 mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer +
B1/2 Esophageal Cancer +
C1/2 Esophagus +
D1/2 Esophagus +
E1/2 Stomach Cancer +
F1/2 Stomach Cancer +
G1/2 Stomach -
H1/2 Stomach -
A3/4 Colon Cancer +
B3/4 Colon Cancer +
C3/4 Colon +
D3/4 Duodenum +
E3/4 Rectal Cancer +
F3/4 Rectal Cancer +
G3/4 Rectum +
H3/4 Testis +
A5/6 Hepatic Carcinoma +
B5/6 Hepatic Carcinoma +
C5/6 Liver +
D5/6 Renal cell Carcinoma -
E5/6 Kidney +
F5/6 Pancreas +
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue +
A7/8 Lung Cancer +
B7/8 Lung Cancer +
C7/8 Lung +
D7/8 Brain -
E7/8 Brain +
F7/8 Medulla oblongata +
G7/8 Cerebellar +
H7/8 Skeletal muscle +
A9/10 Breast Cancer +
B9/10 Breast Cancer +
C9/10 Ovarian Cancer +
D9/10 Ovarian Cancer +
E9/10 Endometrial Cancer +
F9/10 Cervical Cancer +
G9/10 Bladder Cancer +
H9/10 Myocardial +
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