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UHRF1

Order Information
Catelog# Size/Concentration Price
31124 1.0mg/ml $341.00
Specification
Entrez GeneID 29128
Aliases Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95
Clone# 2A8C7
MW 89.8kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human;
Immunogen Purified recombinant fragment of human UHRF1 (AA: 616-755) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
ELISA 1/10000
Description
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.
References
1.Biomarkers. 2015;20(3):183-8.
2.Med Oncol. 2013 Dec;30(4):613.
Sequence
616-755
Related Products

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using UHRF1 mAb against human UHRF1 (AA: 616-755) recombinant protein. (Expected MW is 41.8 kDa)

Western Blot

Figure 3:Western blot analysis using UHRF1 mAb against HEK293 (1) and UHRF1 (AA: 616-755)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using UHRF1 mouse mAb against MCF-7 (1), HCT116 (2), HL-60 (3), Hela (4), and HEK293 (5) cell lysate.

Immunohistochemical analysis

Figure 5:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using UHRF1 mouse mAb with DAB staining.

Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer +
B1/2 Esophageal Cancer +
C1/2 Esophagus -
D1/2 Esophagus +
E1/2 Stomach Cancer -
F1/2 Stomach Cancer -
G1/2 Stomach -
H1/2 Stomach -
A3/4 Colon Cancer +
B3/4 Colon Cancer -
C3/4 Colon +
D3/4 Duodenum -
E3/4 Rectal Cancer -
F3/4 Rectal Cancer +
G3/4 Rectum -
H3/4 Testis -
A5/6 Hepatic Carcinoma -
B5/6 Hepatic Carcinoma +
C5/6 Liver -
D5/6 Renal cell Carcinoma -
E5/6 Kidney -
F5/6 Pancreas -
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue -
A7/8 Lung Cancer -
B7/8 Lung Cancer +
C7/8 Lung -
D7/8 Brain -
E7/8 Brain -
F7/8 Medulla oblongata -
G7/8 Cerebellar -
H7/8 Skeletal muscle -
A9/10 Breast Cancer -
B9/10 Breast Cancer -
C9/10 Ovarian Cancer -
D9/10 Ovarian Cancer -
E9/10 Endometrial Cancer +
F9/10 Cervical Cancer -
G9/10 Bladder Cancer -
H9/10 Myocardial -
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