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PLCG1

Order Information
Catelog# Size/Concentration Price
31122 1.0mg/ml $341.00
Specification
Entrez GeneID 5335
Aliases PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
Clone# 2F3D11
MW 148.5kDa
Host/Isotype Mouse IgG2a
Storage 4℃; -20℃ for long term storage
Species Reactivity Human; Rat;
Immunogen Purified recombinant fragment of human PLCG1 (AA: 39-181) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
FCM 1/200 - 1/400
ELISA 1/10000
Description
The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
References
1.Cancer Discov. 2014 Apr;4(4):OF13.
2.Adv Biol Regul. 2013 Jan;53(1):51-62.
Sequence
39-181
Related Products

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using PLCG1 mAb against human PLCG1 (AA: 39-181) recombinant protein. (Expected MW is 43 kDa)

Western Blot

Figure 3:Western blot analysis using PLCG1 mAb against HEK293 (1) and PLCG1 (AA: 39-181)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using PLCG1 mouse mAb against Jurkat (1), K562 (2), A431 (3), Hela (4), and PC-12 (5) cell lysate.

Flow cytometric

Figure 5:Flow cytometric analysis of Hela cells using PLCG1 mouse mAb (green) and negative control (red).