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Order Information
Catelog# Size/Concentration Price
31101 1.0mg/ml $341.00
Entrez GeneID 57534
Aliases MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2
Clone# 2A7B1
MW 110kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human; Monkey;
Immunogen Purified recombinant fragment of human MIB1 (AA: 6-221) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
WB 1/500 - 1/2000
FCM 1/200 - 1/400
ELISA 1/10000
This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1).
1.J Cell Sci. 2015 May 1;128(9):1674-82.
2.Cell Res. 2012 Mar;22(3):603-6.
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Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using MIB1 mAb against human MIB1 (AA: 6-221) recombinant protein. (Expected MW is 50.1 kDa)

Western Blot

Figure 3:Western blot analysis using MIB1 mAb against HEK293 (1) and MIB1 (AA: 6-221)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using MIB1 mouse mAb against Hela (1) and COS7 (2) cell lysate.

Flow cytometric

Figure 5:Flow cytometric analysis of Hela cells using MIB1 mouse mAb (green) and negative control (red).