Mouse Monoclonal Antibody to LSD1/AOF2
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| Catalog# |
20055 Mab-2005126
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| Clone# |
1B2E5
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human; Mouse; Monkey
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Lot# |
0601
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| Size |
0.1ml
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Price |
$
260
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| MW |
93kDa
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Entrez Gene |
23028
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| Aliases |
KDM1; LSD1; AOF2
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| Description |
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The amine oxidase domain 2 (AOF2) gene encodes a nuclear protein (LSD1, ~95kDa) containing a Swirm domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. LSD1 is a chromatin-modifying enzyme, which serve as a docking module for the stabilization of the associated corepressor complex (es) on chromatin.
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| Immunogen |
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Purified recombinant fragment of human LSD1 expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Shi YJ, et.al Mol Cell. 2005 Sep 16;19(6):857-64.
2. Metzger E, et.al Nature. 2005 Sep 15;437(7057):436-9.
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Western Blotting
Figure 1: Western blot analysis using anti-LSD1 monoclonal antiobdy against COS (1), Hela (2), NIH/3T3(3), A549 (4) and Jurkat (5) cell lysate.
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ICC
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma(left) and kidney carcinoma(right), showing nuclear localization using anti-LSD1 antibody with DAB staining.
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| FOR RESEARCH USE ONLY |
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