Mouse Monoclonal Antibody to VCAM1
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| Catalog# |
30045
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| Clone# |
6G9
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human
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Lot# |
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| Size |
0.1ml
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Price |
$
280
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| MW |
81kDa
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Entrez Gene |
7412
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| Aliases |
CD106; MGC99561; INCAM-100; DKFZp779G2333; VCAM1
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| Description |
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This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.Tissue specificity: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.INVITROGEN: CD106 is expressed on bone marrow stromal cells, myeloid cells, and endothelial cells.
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| Immunogen |
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Purified recombinant fragment of human VCAM1 expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Cell Adh Migr. 2009 Oct;3(4):369-72.
2. Arthritis Rheum. 2010 Jan;62(1):105-16.
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Western Blotting
Figure 1: Western blot analysis using VCAM1 mouse mAb against HUVEC (1) and EC (2) cell lysate.
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IHC-P (paraffin)
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and colon cancer (B) using VCAM1 mouse mAb with DAB staining.
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| FOR RESEARCH USE ONLY |
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