Mouse Monoclonal Antibody to CDH2
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| Catalog# |
30042
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| Clone# |
5D5
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human; Mouse; Rat
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Lot# |
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| Size |
0.1ml
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Price |
$
280
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| MW |
99.8kDa
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Entrez Gene |
1000
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| Aliases |
CDHN; NCAD; CD325; CDw325; CDH2
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| Description |
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This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
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| Immunogen |
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Purified recombinant fragment of human CDH2 expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. J Biol Chem. 2007 Mar 16;282(11):8545-56.
2. Mol Cell Biochem. 2007 Aug;302(1-2):19-26.
3. Urol Oncol. 2010 Mar-Apr;28(2):180-8.
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Western Blotting
Figure 1: Western blot analysis using CDH2 mouse mAb against A431 (1), NIH/3T3 (2), Hela (3), C6 (4) and LNCap(5) cell lysate.
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IHC-P (paraffin)
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), ovarian cancer£¨C) and mammary cancer(D), using CDH2 mouse mAb with DAB staining.
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ICC
Figure 3:Immunofluorescence analysis of A431 cells using CDH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
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FCM
Figure 4: Flow cytometric analysis of PC-2 cells using CDH2 mouse mAb (green) and negative control (purple).
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| FOR RESEARCH USE ONLY |
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