Mouse Monoclonal Antibody to CDC2
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| Catalog# |
30040
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| Clone# |
8C5A7F10
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human
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Lot# |
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| Size |
0.1ml
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Price |
$
280
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| MW |
34kDa
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Entrez Gene |
983
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| Aliases |
CDC2; CDC28A; P34CDC2; MGC111195; DKFZp686L20222; CDK1
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| Description |
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The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.
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| Immunogen |
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Purified recombinant fragment of CDC2 expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Mol Biol Cell. 2008 Aug;19(8):3536-43.
2. Eur J Cancer. 2009 May;45(8):1466-73.
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Western Blotting
Figure 1: Western blot analysis using CDC2 mouse mAb against Jurkat(1) cell lysate.
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ICC
Figure 2:Immunofluorescence analysis of Hela cells using CDC2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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FCM
Figure 3: Flow cytometric analysis of PC-2 cells using CDC2 mouse mAb (green) and negative control (purple).
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| FOR RESEARCH USE ONLY |
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