Mouse Monoclonal Antibody to CD3E
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|
| Catalog# |
30036
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| Clone# |
4E2
|
| Host and isotype |
Mouse IgG1
|
| Species reactivity |
Human
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Lot# |
|
| Size |
0.1ml
|
Price |
$
280
|
| MW |
23kDa
|
Entrez Gene |
916
|
| Aliases |
T3E; TCRE; FLJ18683; CD3E
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| Description |
|
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
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| Immunogen |
|
Purified recombinant fragment of CD3E expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
|
Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Nat Methods. 2005 Aug;2(8):591-8.
2. J Cell Physiol. 2006 Dec;209(3):695-700. Review.
3. J Biol Chem. 2006 Dec 1;281(48):36977-84. Epub 2006 Oct 5.
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Western Blotting
Figure 1: Western blot analysis using CD3E mouse mAb against Jurkat (1) cell lysate.
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FCM
Figure 2: Flow cytometric analysis of Jurkat cells using CD3E mouse mAb (green) and negative control (purple).
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| FOR RESEARCH USE ONLY |
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