Mouse Monoclonal Antibody to CLOCK
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| Catalog# |
30034
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| Clone# |
8F7
|
| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human
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Lot# |
|
| Size |
0.1ml
|
Price |
$
240
|
| MW |
95kDa
|
Entrez Gene |
9575
|
| Aliases |
KAT13D; bHLHe8; KIAA0334; CLOCK
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| Description |
|
This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.
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| Immunogen |
Purified recombinant fragment of human CLOCK expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
Ascitic fluid containing 0.03% sodium azide.
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| Storage |
|
Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Chronobiol Int. 2007;24(4):589-97.
2. Neurosci Lett. 2008 Apr 11;435(1):30-3.
3. Virchows Arch. 2009 Apr;454(4):467-74.
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Western Blotting
Figure 1: Western blot analysis using CLOCK mouse mAb against CLOCK-hIgGFc transfected HEK293 cell lysate.
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ICC
Figure 2:Immunofluorescence analysis of Hela cells using CLOCK mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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| FOR RESEARCH USE ONLY |
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