Mouse Monoclonal Antibody to CD19
 |
|
| Catalog# |
30039
|
| Clone# |
2E2
|
| Host and isotype |
Mouse IgG1
|
| Species reactivity |
Human
|
Lot# |
|
| Size |
0.1ml
|
Price |
$
280
|
| MW |
61kDa
|
Entrez Gene |
930
|
| Aliases |
B4; MGC12802
|
| Description |
|
The CD19 antigen (95kDa) is expressed from the earliest stage of B progenitor development, on all peripheral B cells including germinal centre B cells, and all B cell lines and B cell leukaemia tested. T cell and monocytic cell lines are negative and the antigen is lost on B cell maturation to plasma cells. The antigen is a type I integral membrane glycoprotein whose in vitro inhibition will influence B cell activation and proliferation.
|
| Immunogen |
|
Purified recombinant fragment of human CD19 expressed in E. Coli.
|
| Applications |
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|
| Formulation |
|
Ascitic fluid containing 0.03% sodium azide.
|
| Storage |
|
Store at 4¡æ, for long term storage, store at -20¡æ.
|
| Related product |
|
|
| References |
1. Rie, M.A. de, J. of Immunol. Methods, 1987. 102: 187.
2. Rie, M.A. de, Leukaemia Research, 1988. 12: 135.
|
FCM
Figure 1: Flow cytometric analysis of Raji cells using CD19 mouse mAb (green) and negative control (purple).
|
ICC
Figure 2:Immunofluorescence analysis of HL-60(left) and K562(right) cells using CD19 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
|
|
| FOR RESEARCH USE ONLY |
Product
Category
