Mouse Monoclonal Antibody to CDC25C
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|
| Catalog# |
30022
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| Clone# |
1F12
|
| Host and isotype |
Mouse IgG1
|
| Species reactivity |
Human
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Lot# |
|
| Size |
0.1ml
|
Price |
$
280
|
| MW |
58kDa
|
Entrez Gene |
995
|
| Aliases |
CDC25; CDC25C
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| Description |
|
Cdc25C is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It has been highly conserved during evolution and it plays a key role in the regulation of cell division. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Cdc25C is mainly expressed in G2 phase. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.
|
| Immunogen |
Purified recombinant fragment of human CDC25C expressed in E. Coli.
|
| Applications |
Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|
| Formulation |
Ascitic fluid containing 0.03% sodium azide.
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| Storage |
|
Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Cancer Cell. 2007 Mar;11(3):275-89.
2. Int J Biochem Cell Biol. 2007;39(9):1707-13.
3. Int J Cancer. 2010 May 1;126(9):2199-210.
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Western Blotting
Figure 1: Western blot analysis using anti-CDC25C mAb against Hela(1), K562(2), PC-3(3), HEK293(4) and Raw264.7(5) cell lysate.
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|
| FOR RESEARCH USE ONLY |
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