Mouse Monoclonal Antibody to EGF
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| Catalog# |
30015
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| Clone# |
4E11
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human
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Lot# |
|
| Size |
0.1ml
|
Price |
$
280
|
| MW |
134kDa
|
Entrez Gene |
1950
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| Aliases |
URG; HOMG4; EGF
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| Description |
|
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
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| Immunogen |
Purified recombinant fragment of human EGF expressed in E. Coli.
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| Applications |
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Biochem J. 1992 Dec 1;288 ( Pt 2):395-405.
2. Oncogene. 2000 Mar 16;19(12):1509-18.
3. Nature. 2002 Mar 14;416(6877):183-7.
4. Radiat Res. 2003 Apr;159(4):439-52.
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Western Blotting
Figure 1: Western blot analysis using EGF mAb against EGF-hIgGFc transfected HEK293 cell lysate.
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ICC
Figure 2: Immunofluorescence analysis of NIH-3T3 cells using EGF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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| FOR RESEARCH USE ONLY |
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