Mouse Monoclonal Antibody to Pirh2
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| Catalog# |
30005
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| Clone# |
1H10
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human; Rat
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Lot# |
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| Size |
0.1ml
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Price |
$
280
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| MW |
30kDa; 60kDa (homodimer)
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Entrez Gene |
25898
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| Aliases |
ARNIP; CHIMP; RNF199; RCHY1
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| Description |
|
Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.
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| Immunogen |
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Purified recombinant fragment of human Pirh2 expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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1. #20338, Mouse Monoclonal Antibody to p53.
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| References |
1. Biochem Biophys Res Commun. 2007 Dec 14;364(2):344-50.
2. J Natl Cancer Inst. 2004 Nov 17;96(22):1718-21.
3. Exp Cell Res. 2006 Oct 15;312(17):3370-8.
4. Cell. 2003 Mar 21;112(6):779-91.
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Western Blotting
Figure 1: Western blot analysis using Pirh2 mouse mAb against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.
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ICC
Figure 2: Immunofluorescence analysis of Hela cells using Pirh2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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FCM
Figure 3: Flow cytometric analysis of PC-12 cells using anti-Pirh2 mAb (blue) and negative control (red).
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| FOR RESEARCH USE ONLY |
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