Mouse Monoclonal Antibody to GOT2
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|
| Catalog# |
30004
|
| Clone# |
3E9
|
| Host and isotype |
Mouse IgG1
|
| Species reactivity |
Human; Mouse; Rat; Monkey
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Lot# |
|
| Size |
0.1ml
|
Price |
$
280
|
| MW |
47kDa
|
Entrez Gene |
2806
|
| Aliases |
KAT4; KATIV; mitAAT
|
| Description |
|
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and
inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the
urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
|
| Immunogen |
|
Purified recombinant fragment of human GOT2 expressed in E. Coli.
|
| Applications |
Western Bloting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|
| Formulation |
|
Ascitic fluid containing 0.03% sodium azide.
|
| Storage |
|
Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
|
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| References |
1. Hepatology. 1998 Apr;27(4):1064-74.
2. Cell. 2005 Sep 23;122(6):957-68.
3. Psychiatr Genet. 2007 Oct;17(5):314.
|
Western Blotting
Figure 1: Western blot analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.
|
ICC
Figure 2:Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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|
| FOR RESEARCH USE ONLY |
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