Mouse Monoclonal Antibody to BDH1
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| Catalog# |
30003
|
| Clone# |
1A5
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| Host and isotype |
Mouse IgG1
|
| Species reactivity |
Human; Mouse
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Lot# |
|
| Size |
0.1ml
|
Price |
$
280
|
| MW |
38kDa
|
Entrez Gene |
622
|
| Aliases |
BDH
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| Description |
|
BDH1 (3-hydroxybutyrate dehydrogenase, type 1), it is a member of the short-chain dehydrogenase/reductase gene family. This protien forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. It catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism.
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| Immunogen |
|
Purified recombinant fragment of human BDH1 expressed in E. Coli.
|
| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
|
Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Biochemistry. 1996 Jun 25;35(25):8158-65.
2. Biochemistry. 2000 Oct 3;39(39):11928-38.
3. Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15089-94.
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Western Blotting
Figure 1: Western blot analysis using BDH1 mouse mAb against HepG2 (1) and NIH/3T3 (2) cell lysate.
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IHC-P (paraffin)
Figure 3: Immunohistochemical analysis of paraffin-embedded human liver cancer (left) and colorectal cancer tissues (right) using BDH1 mouse mAb with DAB staining.
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| FOR RESEARCH USE ONLY |
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