Mouse Monoclonal Antibody to ATP2C1
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| Catalog# |
30001
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| Clone# |
4G12
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human; Monkey
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Lot# |
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| Size |
0.1ml
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Price |
$
280
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| MW |
100kDa
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Entrez Gene |
27032
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| Aliases |
HHD; BCPM; PMR1; SPCA1
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| Description |
|
ATP2C1, also known as PMR1, it belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium. The human homologue, ATP2C1 (also
designated SPLA in rat), also regulates the transport of calcium in the Golgi
complex and is related to other P-type ATPases family members, such as the
sarco(endo)plasmic calcium ATPase (SERCA) and the plasma membrane calcium
ATPase (PCMA). ATP2C1 is a transmembrane protein that exists as two
splice variants, which vary by 20 amino acids. Defects in ATP2C1 cause Hailey-Hailey disease, which is an autosomal dominant disorder that is characterized by blisters and erosions of the skin. These findings provide further evidence that PMR1 plays a key role in maintaining the integrity of the epidermis by controlling intracellular calcium signaling.
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| Immunogen |
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Purified recombinant fragment of ATP2C1 expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. J Invest Dermatol. 2005 Nov;125(5):933-5.
2. J Dermatol Sci. 2006 Aug;43(2):150-1.
3. Dermatology. 2007;215(4):277-83.
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Western Blotting
Figure 1: Western blot analysis using ATP2C1 mouse mAb against human recombinant ATP2C1.
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Western Blotting
Figure 2: Western blot analysis using ATP2C1 mouse mAb against A431 (1), Hela (2) and HEK293 (3) cell lysate.
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IHC-P (paraffin)
Figure 3: Immunohistochemical analysis of paraffin-embedded human ovarian cancer (left) and breast cancer (right) tissues using ATP2C1 mouse mAb with DAB staining.
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| FOR RESEARCH USE ONLY |
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