Mouse Monoclonal Antibody to Metadherin
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| Catalog# |
20407
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| Clone# |
2F11C3
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human
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Lot# |
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| Size |
0.1ml
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Price |
$
280
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| MW |
64kDa
|
Entrez Gene |
92140
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| Aliases |
3D3; AEG1; LYRIC; MTDH
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| Description |
|
Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.
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| Immunogen |
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Purified recombinant fragment of human MTDH expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Determining optimal working dilutions by titration test.
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| Formulation |
Antibody are purified by protein G affinity chromatography.
Liquid in PBS containing 50% glycerol and 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Cancer Cell. 2004 Apr;5(4):365-74.
2. Exp Cell Res. 2004 Oct 15;300(1):134-48.
3. Cancer Cell. 2009 Jan 6;15(1):9-20.
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Western Blotting
Figure 1: Western blot analysis using anti-Metadherin mAb against K562 (1), SKBR-3 (2), T47D (3), Hela (4) and MCF-7 (5) cell lysate.
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IHC-P (paraffin)
Figure 2: Immunohistochemical analysis of paraffin-embedded human liver cancer(A) and brain tissue(B), showing cytoplasmic localization using anti-Metadherin antibody with DAB staining.
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FCM
Figure 3: Flow cytometric analysis of Hela cells using anti-Metadherin mAb (green) and negative control (purple).
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| FOR RESEARCH USE ONLY |
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