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Products
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Mouse Monoclonal Antibody to NF-百B p65
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| Catalog# |
20406
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| Clone# |
6H7
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human; Mouse
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Lot# |
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| Size |
0.1ml
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Price |
$
280
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| MW |
65kDa
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Entrez Gene |
5970
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| Aliases |
NFkappaB p65; p65; NFKB3; RELA
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| Description |
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Transcription factors of the nuclear factor 百 B (NF-百B)/Rel family is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. There are five family members in mammals: RelA (p65), c-Rel, RelB, NF-百B1 (p105/p50) and NF-百B2 (p100/p52). The most studied NF-百B complex consists of the p50 and p65 subunits, both containing a 300 amino acid region with homology to the Rel proto-oncogene product. The p50 subunit binds DNA, whereas the p65 subunit is responsible for the
interaction of NF-百B with its inhibitor, I百B. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to I百B. This complex prevents nuclear translocation and activity of NF-百B. In response to stimuli such as cytokines, LPS, and viral infections, I百B is phosphorylated at critical residues. This phosphorylation induces dissociation of the I百B/NF-百B complex, allowing the free heterodimeric NF-百B to form a heterotetramer that translocates to the nucleus. In the nucleus, it binds to the 百B site within promoters and enhancers and functions as a transcriptional activator.
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| Immunogen |
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Purified recombinant fragment of human NF-百B p65 expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4⊥, for long term storage, store at -20⊥.
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| Related product |
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| References |
1. Nature. 1997 Aug 7;388(6642):548-54.
2. Cell. 1998 Dec 11;95(6):749-58.
3. J Biol Chem. 2000 Jun 16;275(24):18180-7.
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Western Blotting
Figure 1: Western blot analysis using anti-NF-百B p65 mAb against Jurkat (1), K562 (2) and NIH/3T3 (3) cell lysate.
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| FOR RESEARCH USE ONLY |
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