Mouse Monoclonal Antibody to AFP
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| Catalog# |
20405
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| Clone# |
6E6
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| Host and isotype |
Mouse IgG2b
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| Species reactivity |
Human
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Lot# |
|
| Size |
0.1ml
|
Price |
$
240
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| MW |
69kDa
|
Entrez Gene |
174
|
| Aliases |
alpha-fetoprotein; FETA; HPAFP
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| Description |
|
AFP (alpha-fetoprotein), a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
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| Immunogen |
|
Purified recombinant fragment of human AFP expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
Antibody are purified by protein G affinity chromatography.
Liquid in PBS containing 50% glycerol and 0.03% sodium azide.
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| Storage |
|
Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7160-4.
2. Pediatr Hematol Oncol. 2001 Dec;18(8):509-18.
3. Tumour Biol. 2002 Jul-Aug;23(4):202-11.
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Western Blotting
Figure 1: Western blot analysis using anti-AFP mAb against HepG2 (1) and SMMC-7721 (2) cell lysate.
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| FOR RESEARCH USE ONLY |
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