Products

Mouse Monoclonal Antibody to eNOS             
 Catalog#   20397   
 Clone#   6H2
 Host and isotype   Mouse IgG1
 Species reactivity   Human  Lot#  
 Size   0.1ml  Price  $ 280
 MW   133kDa  Entrez Gene   4846
 Aliases   ECNOS; NOS3
 Description
Endothelial nitric-oxide synthase (eNOS), also known as NOS3, it is an important enzyme in the cardiovascular system. It is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm.
 Immunogen
Purified recombinant fragment of human eNOS expressed in E. Coli.
 Applications
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
 Formulation
Ascitic fluid containing 0.03% sodium azide.
 Storage
Store at 4¡æ, for long term storage, store at -20¡æ.
 Related product
 References
1. Nature. 1999 Jun 10;399(6736):601-5.
2. Oncol Rep. 2004 Nov;12(5):1007-11.
3. Breast Cancer Res Treat. 2008 May;109(1):181-2.

IHC-P (paraffin)
Figure 1: Immunohistochemical analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues using anti-eNOS antibody with DAB staining.
IHC-P (paraffin)
Figure 2: Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and ovary cancer (right) tissues using anti-eNOS antibody with DAB staining.

FOR RESEARCH USE ONLY
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