Mouse Monoclonal Antibody to WNT10B
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| Catalog# |
20395 Mab-20091017
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| Clone# |
5A7
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human
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Lot# |
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| Size |
0.1ml
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Price |
$
320
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| MW |
43kDa
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Entrez Gene |
7480
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| Aliases |
SHFM6; WNT-12
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| Description |
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WNT10B: wingless-type MMTV integration site family, member 10B. The WNT family consists of structurally related secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is ikely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10B protein at the amino acid level. The WNT10B gene is clustered with another family member, WNT1, in the chromosome 12q13 region.
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| Immunogen |
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Purified recombinant fragment of human WNT10B expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Oncogene. 1997 Mar 13;14(10):1249-53.
2. Int J Oncol. 2001 Dec;19(6):1187-92
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Western Blotting
Figure 1: Western blot analysis using anti-WNT10B mAb against Hela cell lysate (1).
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IHC-P (paraffin)
Figure 2: Immunohistochemical analysis of paraffin-embedded human normal stomach (A), normal liver (B), normal kidney (C) and rectum cancer tissues (D) using anti-WNT10B mAb with DAB staining.
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ICC
Figure 3: Confocal immunofluorescence analysis of PANC-1 cells using anti-WNT10B mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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| FOR RESEARCH USE ONLY |
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