Mouse Monoclonal Antibody to WNT1
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| Catalog# |
20378 Mab-20090925
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| Clone# |
10C8
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human; Mouse
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Lot# |
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| Size |
0.1ml
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Price |
$
280
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| MW |
41kDa
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Entrez Gene |
7471
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| Aliases |
INT1
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| Description |
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WNT1: wingless-type MMTV integration site family, member 1. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant rolein Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.
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| Immunogen |
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Purified recombinant fragment of WNT1 expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Blood. 2008 Jan 1;111(1):122-31.
2. BMC Cancer. 2005 May 24;5:53.
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Western Blotting
Figure 1: Western blot analysis using anti-WNT1 monoclonal antibody against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.
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ICC
Figure 2: Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using anti-WNT1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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FCM
Figure 4: Flow cytometric analysis of Hela cells using anti-WNT1 mAb (green) and negative control (purple).
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| FOR RESEARCH USE ONLY |
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