Mouse Monoclonal Antibody to CK17
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| Catalog# |
20363 Mab-20090910
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| Clone# |
3B12
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| Host and isotype |
Mouse IgG2b
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| Species reactivity |
Human
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Lot# |
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| Size |
0.1ml
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Price |
$
280
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| MW |
49kDa
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Entrez Gene |
3872
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| Aliases |
PC; K17; PC2; PCHC1; KRT17
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| Description |
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CK17, also known as KRT17, it is the type I intermediate filament chain keratin 17. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells". May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation. Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state. Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway. Involved in tissue repair.
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| Immunogen |
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Purified recombinant fragment of CK17 expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Am J Pathol. 2002 Dec;161(6):1991-6.
2. Br J Dermatol. 1995 Oct;133(4):501-11.
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Western Blotting
Figure 1: Western blot analysis using anti-CK17 monoclonal antibody against Hela (1), MCF-7 (2) and A431 (3) cell lysate.
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IHC-P (paraffin)
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), endometrial carcinoma (B), sublingual gland (C) and esophagus (D) tissues using anti-CK17 antibody with DAB staining.
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| FOR RESEARCH USE ONLY |
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