Mouse Monoclonal Antibody to PAK2
 |
|
| Catalog# |
20356 Mab-2009093
|
| Clone# |
3B5
|
| Host and isotype |
Mouse IgG1
|
| Species reactivity |
Human; Monkey
|
Lot# |
|
| Size |
0.1ml
|
Price |
$
329
|
| MW |
61kDa
|
Entrez Gene |
5062
|
| Aliases |
PAK65; PAKgamma
|
| Description |
|
PAK2, also known as P21 (CDKN1A)-activated kinase 2. The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. PAK2 is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. PAK2 has been shown to interact with SH3KBP1, CDC42 and Abl gene.
|
| Immunogen |
|
Purified recombinant fragment of PAK2 expressed in E. Coli.
|
| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|
| Formulation |
|
Ascitic fluid containing 0.03% sodium azide.
|
| Storage |
|
Store at 4¡æ, for long term storage, store at -20¡æ.
|
| Related product |
|
|
| References |
1. J Immunol. 2004 Jun 15;172(12):7324-34.
2. J Mol Biol. 2007 Jul 20;370(4):620-32.
|
Western Blotting
Figure 1: Western blot analysis using anti-PAK2 monoclonal antibody against Hela (1), Jurkat (2), A549 (3), HEK293 (4) and K562 (5) cell lysate.
|
IHC-P (paraffin)
Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and gastric cancer (right) using anti-PAK2 antibody with DAB staining.
|
ICC
Figure 3: Confocal immunofluorescence analysis of Hela cells using anti-PAK2 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
|
|
|
| FOR RESEARCH USE ONLY |
Product
Category
