Mouse Monoclonal Antibody to MYL3
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| Catalog# |
20343 Mab-2009073
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| Clone# |
7C1
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human
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Lot# |
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| Size |
0.1ml
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Price |
$
320
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| MW |
22kDa
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Entrez Gene |
4634
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| Aliases |
CMH8; VLC1; MLC1V; MLC1SB
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| Description |
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Myosins are a large superfamily of motor proteins that move along actin filaments, while hydrolyzing ATP. Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains and four light chains. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Human myosin light chain has clinical application as a cardiac marker. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy.
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| Immunogen |
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Purified recombinant fragment of MYL3 expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
1. #20342, Mouse Monoclonal Antibody to MYL3;
2. #20324, Mouse Monoclonal Antibody to MYL2.
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| References |
1. Xie B, et al. Biophys Chem. 2003 Oct 1;106(1):57-66.
2. Haase H, et al. FASEB J. 2006 May;20(7):865-73.
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Western Blotting
Figure 1: Western blot analysis using anti-MYL3 (1) and anti-MYL2 (2) monoclonal antibody against rat fetal heart tissues lysate.
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IHC-P (paraffin)
Figure 2: Immunohistochemical analysis of paraffin-embedded human skeletal muscle(left) and cardiac muscle(right) using anti-MYL3 antibody with DAB staining.
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| FOR RESEARCH USE ONLY |
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