Mouse Monoclonal Antibody to NANOG
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| Catalog# |
20289 Mab-2009011
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| Clone# |
1E6C4
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human
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Lot# |
|
| Size |
0.1ml
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Price |
$
280
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| MW |
35kDa
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Entrez Gene |
79923
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| Aliases |
NANOG
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| Description |
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NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.
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| Immunogen |
Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
1. #20203, Mouse Monoclonal Antioby to cMYC;
2. #20271, Mouse Monoclonal Antioby to LIN28.
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| References |
1. Genes Cells. 2006 Sep;11(9):1115-23.
2. Mol Biol Cell. 2007 May;18(5):1543-53.
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Western Blotting
Figure 1: Western blot analysis using anti-NANOG monoclonal antibody against NTERA-2 cell lysate (2).
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ICC
Figure 2: Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using anti-Nanog Mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
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| FOR RESEARCH USE ONLY |
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