Mouse Monoclonal Antibody to Cytokeratin 8
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| Catalog# |
20280 Mab-2008122
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| Clone# |
8A5D12
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human
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Lot# |
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| Size |
0.1ml
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Price |
$
320
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| MW |
54kDa
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Entrez Gene |
3856
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| Aliases |
CK8; CYK8; K2C8; KRT8
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| Description |
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Cytokeratin 8, also known as CK8, KRT8, K8. Entrez Protein NP_002264. It is a member of the type II keratin family.Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells.It typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
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| Immunogen |
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Purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
1. #20276, Mouse Monoclonal Antibody to Cytokeratin 18;
2. #20277, Mouse Monoclonal Antibody to Cytokeratin 18;
3. #20283, Mouse Monoclonal Antibody to Cytokeratin 19.
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| References |
1. Cancer Genet Cytogenet. 2007 Oct 15;178(2):94-103.
2. Cancer Lett. 2008 Jul 8;265(2):188-96.
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Western Blotting
Figure 1: Western blot analysis using anti-CK8 monoclonal antibody against A549 (1), Hela (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate.
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IHC-P (paraffin)
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma(A), lung cancer(B) and ovarian cancer tissue(C), showing membrane and cytoplasmic localization with DAB staining using anti-CK8 antibody.
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ICC
Figure 3: Confocal immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using anti-CK8 monoclonal antioby (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
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| FOR RESEARCH USE ONLY |
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