Mouse Monoclonal Antibody to LPP
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| Catalog# |
20178 Mab-2007129
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| Clone# |
8B3A11
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human; Mouse; Monkey; Hamster
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Lot# |
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| Size |
0.1ml
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Price |
$
320
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| MW |
66kDa
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Entrez Gene |
4026
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| Aliases |
LPP
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| Description |
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LIM domain containing preferred translocation partner in lipoma. The Zyxin family of proteins contains five members, Ajuba, LIMD1, LPP,TRIP6 and Zyxin. LPP (LIM-containing lipoma-preferred partner), a LIM domain-containing scaffolding protein contains three LIM domains at its carboxy
terminus, which are preceded by a proline-rich pre-LIM region containing a number of protein interaction domains. LPP, an 80 kDa protein, localizes to sites of cell adhesion, such as focal adhesions and cell-cell contacts,and shuttles to the nucleus where it has transcriptional activation capacity.The human LPP gene maps to chromosomal location 3q28, and preferentially
translocates to the HMGIC gene in a subclass of human benign mesenchymal tumors known as lipomas.
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| Immunogen |
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Purified recombinant fragment of human LPP expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. BMC Cell Biol. 2005 Jan 13;6(1):1
2. Mol Cell Proteomics. 2005 Sep;4(9):1240-50. Epub 2005 Jun 11.
3. Cancer Genet Cytogenet. 2005 Nov;163(1):68-70.
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Western Blotting
Figure 1: Western blot analysis using anti-LPP mAb against Hela (1), NIH/3T3 (2), COS (3), Caki (4), MCF-7 (5), HepG2 (6) and SMMC-7721 (7) cell lysate.
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IHC-P (paraffin)
Figure 2: Immunohistochemical analysis of paraffin-embedded human small intestine using anti-LPP mAb with DAB staining.
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IHC-P (paraffin)
Figure 3: Immunohistochemical analysis of paraffin-embedded human vessels tissues using anti-LPP mAb.
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ICC
Figure 4: Confocal immunofluorescence analysis of COS cells using anti-LPP mAb (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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ICC
Figure 5: Confocal immunofluorescence analysis of Hela cells using anti-LPP mAb (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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| FOR RESEARCH USE ONLY |
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