Mouse Monoclonal antibody to Nucleophosmin
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| Catalog# |
20001 Mab-2004001
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| Clone# |
7H10B9
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human; Monkey
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Lot# |
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| Size |
0.1ml
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Price |
$
240
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| MW |
33kDa
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Entrez Gene |
4869
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| Aliases |
B23; NPM
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| Description |
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Nucleophosmin (NPM), also named B23 or NO38, is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. NPM has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities . The NPM is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.
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| Immunogen |
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Purified recombinant fragment of human NPM expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Morris SW, et al Science 1994 Mar 4; 263(5151):1281-4.
2. Yoneda-Kato N Oncogene 1996 Jan 18;12(2):265-75.
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Western Blotting
Figure 1: Western blot analysis using anti-NPM mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.
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IHC-P (paraffin)
Figure 2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissues, showing nuclear localization using anti-NPM mAb with DAB staining.
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ICC
Figure 3: Confocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using anti-NPM mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
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| FOR RESEARCH USE ONLY |
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