Mouse Monoclonal Antibody to CD44
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| Catalog# |
20282 Mab-2008124
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| Clone# |
8E2F3
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| Host and isotype |
Mouse IgG1
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| Species reactivity |
Human, Mouse
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Lot# |
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| Size |
0.1ml
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Price |
$
240
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| MW |
82kDa
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Entrez Gene |
960
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| Aliases |
IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL
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| Description |
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CD44, also known as IN, LHRMIC4, CDW44, HCELL. It is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
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| Immunogen |
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Purified recombinant fragment of human CD44 (628-699) expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Exp Mol Pathol. 2007 Dec;83(3):341-6.
2. Mol Cell Proteomics. 2008 Mar;7(3):499-508.
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Western Blotting
Figure 1: Western blot analysis using anti-CD44 mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).
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Western Blotting
Figure 2: Western blot analysis using anti-CD44 mAb against Hela (1) and HUVE-12(2) cell lysate.
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IHC-P (paraffin)
Figure 3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using anti-CD44 mAb.
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IHC-P (paraffin)
Figure 4: Confocal analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
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ICC
Figure 5: Confocal immunofluorescence analysis of PANC-1 cells using anti-CD44 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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ICC
Figure 6: Confocal immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
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FCM
Figure 7: Flow cytometric analysis of Hela cells using anti-CD44 mAb (right) and negative control (left).
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| FOR RESEARCH USE ONLY |
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