Mouse Monoclonal Antiobody to MLH1
 |
|
| Catalog# |
20246 Mab-2008088
|
| Clone# |
4C9C7
|
| Host and isotype |
Mouse IgG1
|
| Species reactivity |
Human; Monkey
|
Lot# |
|
| Size |
0.1ml
|
Price |
$
280
|
| MW |
85kDa
|
Entrez Gene |
4292
|
| Aliases |
FCC2; COCA2; HNPCC
|
| Description |
|
DNA-mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. Lack of a functional DNA-mismatch repair pathway is a common
characteristic of several different types of human cancers, either due to an
MMR gene mutation or promoter-methylation gene silencing. MLH1 is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in hereditary nonpolyposis colon cancer (HNPCC). MLH1 is an integral
part of the protein complex responsible for mismatch repair expressed in
lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and
gall bladder, and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal
cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Inactivation of the MLH1 gene causes genome instability and predisposition to cancer. MLH1 also plays a role in meiotic recombination.
|
| Immunogen |
Purified recombinant fragment of MLH1 (aa381-483) expressed in E. Coli.
|
| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|
| Formulation |
|
Ascitic fluid containing 0.03% sodium azide.
|
| Storage |
|
Store at 4¡æ, for long term storage, store at -20¡æ.
|
| Related product |
|
1. #20025, Mouse Monoclonal Antibody to MSH2
|
| References |
1. Int J Cancer. 2007 Aug 1;121(3):555-8.
2. Autophagy. 2007 Jul-Aug;3(4):368-70.
3. Fam Cancer. 2008 Jun;7(2):163-172.
|
Western Blotting
Figure 1: Western blot analysis using anti-MLH1 mAb against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.
|
IHC-P (paraffin)
Figure 2: Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using anti-MLH1 mAb.
|
ICC
Figure 3: Confocal immunofluorescence analysis of Hela cells using anti-MLH1 mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
|
|
|
| FOR RESEARCH USE ONLY |
Product
Category
