Mouse Monoclonal Antibody to AMACR
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| Catalog# |
20155 Mab-2007101
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| Clone# |
2A10F3
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| Host and isotype |
Mouse IgG2b
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| Species reactivity |
Human; Mouse
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Lot# |
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| Size |
0.1ml
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Price |
$
340
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| MW |
42kDa
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Entrez Gene |
23600
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| Aliases |
RACE
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| Description |
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AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.
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| Immunogen |
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Purified recombinant fragment of human AMACR expressed in E. Coli.
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| Applications |
Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
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| Formulation |
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Ascitic fluid containing 0.03% sodium azide.
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| Storage |
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Store at 4¡æ, for long term storage, store at -20¡æ.
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| Related product |
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| References |
1. Chen Q. Watson JT. Marengo SR. et al. Cancer Lett. 2006, Dec 8, 244 (2):274-88.Epub 2006 Feb 23.
2. Epstein JI. Herawi M. J Urol. 2006, Mar, 175 (3 Pt 1):820-34. Review.
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Western Blotting
Figure 1: Western blot analysis using anti-AMACR mAb against Jurkat (1) and LNCaP (2) cell lysate.
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IHC-P (paraffin)
Figure 2: Immunohistochemical analysis of paraffin-embedded human normal prostate tissues (left) and prostate adenocarcinoma tissues (right), showing cytoplasmic localization using anti-AMACR mAb with DAB staining.
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IHC-P (paraffin)
Figure 3: Immunohistochemical analysis of paraffin-embedded human brain cerebellum using anti-AMACR mAb.
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ICC
Figure 4: Confocal immunofluorescence analysis of LNCaP cells using anti-AMACR mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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| FOR RESEARCH USE ONLY |
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